专业英语细胞培养.pptx
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1、Cell Culture Maolie liujing Outline nIntroductionnThe Physical Environment of Cell CulturenStandard Cell Culture TechniquesnContamination(污染污染)in Cell CultureintroductionnCell culture is the process by which prokaryotic,eukaryotic or plant cells are grown under controlled conditions.But in practice
2、it refers to the culturing of cells derived from animal cellsnThe History of Cell Culture In 1912,Alexis Carrel,attempted to improve the state of the art of animal cell culture with experiments on the culture of chick embryo tissue.Carrel succeeded in expanding the possibilities of cell culture by k
3、eeping fragments of chick embryo heart alive and beating into the third month of culture and growing chick embryo connective tissue for over 3 months.This,along with Harry Eagles(1955)demonstration that the complex tissue extracts,clots,and so forth previously used to grow cells,opened up a new area
4、 of cell culture.The demonstration that each cell type has an optimal mix of nutrients that supports its function(Ham and McKeehan,1979;Waymouth,1981)has led to media derived to support specific cell types under specialized conditions.Finally,the advent of recombinant expression in mammalian cells a
5、nd the creation of antibody-producing hybridoma(杂交瘤)cell lines,coupled with the use of large-scale culture techniques for culturing mammalian cells.A photograph of an industrial large-scale culture facility used to make recombinant proteins in mammalian cell culture.(A)Primary culture of isolated gr
6、anulosa cells(粒层粒层细胞细胞);(B)a coculture of several cell types from neonatal(新生的新生的)rat lung;(C)a follicle(卵泡卵泡)derived from coculture of rat immature granulosa cells and oocytes(卵母细胞卵母细胞)that reassociate in vitro.The Physical Environment of Cell Culture LaboratorynThe essential idea of the entire pan
7、oply of the chemical solution and mechanical apparatus surrounding the cell in vitro culture is to recreate the physical,nutritional,and hormonal environment of the cell in vivo.nThis includes controlling the temperature,pH,osmolality,and gaseous environment providing a supporting surface,and protec
8、ting the cell from chemical,physical,and mechanical stress.nTemperature Most mammalian cell cultures are grown in incubators that are set at 37C.This was chosen because it is the core body temperature of Homo sapiens.One might therefore expect it to be the optimal temperature for growing many cell l
9、ines derived from human tissues.However,there is no reason to expect 37C to be optimal for all types of human cells,and certainly not for all cells from all mammals.Figure shows packed cell volume(a measure of total cell number)and protein secretion by CHO cells grown at various temperatures.Growing
10、 cells at too high a temperature is more detrimental(有害的有害的)than too low a temperature.npH Most media strive to achieve and maintain a pH between 7.0 and 7.4,with a median of 7.2.Different cell types may have an optimal pH slightly outside this range,and cells certainly differ widely in their abilit
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- 专业 英语 细胞培养
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