多种藻类对As(Ⅲ)的耐受性及吸附研究(英文)_李妍丽.pdf
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1、Study on Tolerance and Biosorption of VariousMicroalgae Species to ArsenicYanli LI,Lin KE*College of Environmental Science and Engineering,South China University of Technology,Guangzhou 510006,ChinaSupported by Natural Science Foundation of Guangdong Province(10151064101000-041);Ph.D.Funds from Mini
2、stry of Education of China(20090172120032).*Corresponding author.E-mail:Received:March 17,2012Accepted:March 30.2012AAgricultural Science&Technology,2012,13(6):1303-1308,1327Copyright 訫 2012,Information Institute of HAAS.All rights reservedResources and EnvironmentAbstractObjective This study aimed
3、to select microalgae species which are capa-ble to effectively remove arsenic contamination from water under natural conditions.Method Four microalgae species Chlorella sp.(zfsaia),Chlorella minata,Chlorella vul-garis and Selenastrum capricormulum were used as experimental materials and cul-tured wi
4、th six different concentrations of As(III)(0.5,1.0,2.0,5.0,10.0,20.0 mg/L).Biomass,chlorophyll a content and other physiological indicators were determined toinvestigate the arsenic tolerance and biosorption of four microalgae species.ResultChlorella sp.is sensitive to arsenic toxicity,its growth wa
5、s inhibited when arsenicconcentration exceeded 10 mg/L,with an EC50of 17.32 mg/L;when the arsenicconcentration was 0-20 mg/L,growth of S.c,ww1 and C.v was not affected,whichshowed relatively high tolerance to arsenic,with arsenic removal rates of 77.02%,72.18%and 81.36%respectively after 24 h.Conclu
6、sion This study indicates thatmicroalgae have good application prospects for processing arsenic wastewater andbeing indicator plants of arsenic wastewater.Key words As(III);Microalgae;Toxicity effect;BiosorptionArsenic is ubiquitous in the soil,sediment,water,atmosphere,and even organisms,which isod
7、orless and colorless,with strongtoxicity.Arsenic contamination in theenvironment poses a serious threat tohuman health,which has been recog-nized as a global public health prob-lem.High levels of arsenic in waterbodies are caused by natural or hu-man activities,such as the geothermalactivities or we
8、athering of ore,mining,manufacturinganduseofarsenicpesticides.Under natural conditions,arsenic has four stable valence statesin different redox environments,in-cluding+5(arsenate),+3(arsenite),0(arsenic)and-3(arsine).The con-centration and existing form of arsenicdetermine its toxicity and removal e
9、ffi-ciency in water treatment.Arseniccontamination of groundwater is themost serious in most basins of MeikeNa-Brahmaputra River in Bangladeshand India,where as many as 500 mil-lion people are exposed to arsenicthreat1.According to statistics,thereare at least three million people livingin arsenic c
10、ontaminated areas in Chi-na,and more than 30 000 patientshave been confirmed arsenic poison-ing2.Specifically,Chia Nan,Kuitun,Hetao and Datong are typical arsenicpoisoning areas caused by drinkingwater3.According to reports,the ar-senic poisoning accident happened inHechi in October 2008 was caused
11、byarsenic contamination of drinking wa-ter,resulting in about 450 victims.A large number of researches arereported on the acute toxicity tests ofheavy metals(Hg,Cu,Cd and As)byusingaquaticorganisms,includingtilapia,carp,protozoa,aquatic vascu-lar plants,etc4-7.However,few resea-rches are reported on
12、 the toxic effectsof arsenic on plant cells,especially onalgae.Algae are very sensitive to thetoxicity of many pollutants8-9.To bespecific,green algae is the simplestandmostprimitivealgaespecies,which plays very important roles forthe balance and stability of aquatic e-cosystems as the primary produ
13、cers ofaquatic ecosystems.Therefore,it isvery necessary to study the feedbackof algae species to arsenic contamina-tion,including their response,adsorp-tion and metabolism to the toxicity ofarsenic.Among heavy metals,toxic ef-fects of Cu on algae are the most in-vestigated10-11.Davies12reported that
14、Dunaliella salina can form giant cellswithout splitting when the Hg concen-tration reaches 10 mg/L.In 1984,Hosea et al.13found that Chlorella vul-garishasahighaffinitytogold.Travieso et al.14reported that thegrowth of Chlorella is not affected un-der 45 mg/L of Cr(VI),while Scenede-smus acuminatus s
15、hows no growthunder Cr concentration higher than 15mg/L.Xiao and Yamaoka et al.15-17found that the toxicity threshold of As(V)is 10-41 mol/dm3to Phaeodactylumtricornutum Bohlin and 10-49 mol/dm3toDirateriainornate.Inaddition,Dunaliella sp.was found to have higharsenic removal efficiency17.Algae test
16、 is integral to the eco-toxicological studies,with short growthcycle,easy isolation and culture,directobservation of poisoning symptomsand other characteristics.Therefore,algae are ideal materials for toxicitytest.In this study,in accordance withthe methods prescribed by the Inter-national Standards
17、 Organization(ISO,2010)18,four microalgae species wereused as experimental biological mate-rials to investigate the toxicity effect ofAs(III)and the adsorption of microal-gae species with high tolerance to ar-senic based on a variety of indicators.Materials and MethodsMaterialsExperimental materials
18、MicroalgaeDOI:10.16175/ki.1009-4229.2012.06.002Agricultural Science&TechnologyAgricultural Science&TechnologyVol.13,No.6,20122012Table 1 Microalgae species used in this experimentNo.Microalgae speciesAbbreviation1Selenastrum capricormulumS.c2Chlorella vulgarisC.v3Chlorella minataww14Chlorella sp.(zf
19、saia)zfsaiaTable 2 Various As(III)concentrations for toxicity testTreatmentFinal concentrationof As(III)mg/LVolume of As(III)lVolume ofmediummlVolume of microalgaesolutionml10020220.512.520231.02520242.05020255.0125202610.0250202720.0500202species used in this experiment wereprovided by the City Uni
20、versity of HongKong,and the specific species areshown in Table 1.Reagents and culture mediumTrivalent arsenic(As2O3)was purcha-sedfrom Shanghai Jingchun ReagentsCo.,Ltd.Experimental drugs used inthis experiment were all of analyticalgrade.Bristol medium(ISO,2004)was adopted in this experiment,con-ta
21、ining 25 g/L of NaNO3,7.5 g/L ofK2HPO4,17.5 g/L of KH2PO4,11.8 g/Lof MgSO47H2O,2.5 g/L of NaCl,2.5 g/L of CaCl22H2O,0.5 g/L ofFeCl36H2O,0.03 g/L of MnCl24H2O,0.002 g/L of CoCl26H2O,0.001 g/L ofCuSO45H2O,0.004g/LofZnSO47H2O,0.002 g/L of NaMoO42H2O and 0.54g/L of EDTA.The cultivation liquid wasprepare
22、d by using pure water,with ini-tial pH of(6.50 0.2).Main equipments and instrumentsMultifunctional Microplate Reader(BiotekServices,Inc.);centrifuge(Heraeus,Germany);desktop freezethermostat oscillator(Shanghai Tai-cang);Vertical Pressure Steam Steril-izer(Shanghai Shenan Medical In-strument Factory
23、);pH meter(OAK-TON,U.S.);hydride generation-atom-ic fluorescence spectrophotometer(Ji-tian AFS-830).To remove external ar-senic and other metal contamination,all glassware used in this experimentwere soaked with 10%nitric acid for24 h,rinsed with ultrapure water,andthen placed in the oven and dried
24、at 80 before use18.MethodsToxicity testToxicant concentra-tions were set in accordance withTable 2,different volumes of As(III)solution was added to the preparedmedium as cultivation liquid for mi-croalgae.Liquid of microalgae at loga-rithmic growth phase was inoculated tocultivation liquid with dif
25、ferent concen-trations of As(III).Sampling was con-ducted every day for 7 d to determinethe growth conditions of microalgae,microalgae liquid without arsenic un-der thesamecultivationconditionswas used as the control.The experiment was carried out in50 ml wide-mouth erlenmeyer bottles.Mouths of all
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